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PubMed · 17013924

Microarray analysis of the developing cortex.

Mawahib O Semeralul·Paul C Boutros·Olga Likhodi·Allan B Okey·Hubert H M Van Tol·Albert H C Wong
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Abstract

Abnormal development of the prefrontal cortex (PFC) is associated with a number of neuropsychiatric disorders that have an onset in childhood or adolescence. Although the basic laminar structure of the PFC is established in utero, extensive remodeling continues into adolescence. To map the overall pattern of changes in cortical gene transcripts during postnatal development, we made serial measurements of mRNA levels in mouse PFC using oligonucleotide microarrays. We observed changes in mRNA transcripts consistent with known postnatal morphological and biochemical events. Overall, most transcripts that changed significantly showed a progressive decrease in abundance after birth, with the majority of change between postnatal weeks 2 and 4. Genes with cell proliferative, cytoskeletal, extracellular matrix, plasma membrane lipid/transport, protein folding, and regulatory functions had decreases in mRNA levels. Quantitative PCR verified the microarray results for six selected genes: DNA methyltransferase 3A (Dnmt3a), procollagen, type III, alpha 1 (Col3a1), solute carrier family 16 (monocarboxylic acid transporters), member 1 (Slc16a1), MARCKS-like 1 (Marcksl1), nidogen 1 (Nid1) and 3-hydroxybutyrate dehydrogenase (heart, mitochondrial) (Bdh).

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Age FactorsAnimalsAnimals, NewbornCluster AnalysisDNA Methyltransferase 3AGene Expression Regulation, DevelopmentalMaleMiceMice, Inbred C57BLMicroarray AnalysisPrefrontal CortexRNA, MessengerReverse Transcriptase Polymerase Chain ReactionJournal ArticleResearch Support, Non-U.S. Gov't

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Mawahib O Semeralul, Paul C Boutros, Olga Likhodi, Allan B Okey, Hubert H M Van Tol, Albert H C Wong. 2006. Microarray analysis of the developing cortex.. https://doi.org/10.1002/neu.20302

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