Blockage of AMV reverse transcriptase by antisense oligodeoxynucleotides.

N Loreau; C Boiziau; P Verspieren; D Shire; J J Toulmé

doi:10.1016/0014-5793(90)81327-k

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Blockage of AMV reverse transcriptase by antisense oligodeoxynucleotides.

N Loreau·C Boiziau·P Verspieren·D Shire·J J Toulmé

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Abstract

Synthetic oligodeoxynucleotides, either unmodified or linked to an intercalating agent, have been used to prevent cDNA elongation by the AMV reverse transcriptase. Oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA part bound to the antisense oligonucleotide by the RNase-H activity associated with the retroviral polymerase.

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Animals Avian Myeloblastosis Virus Base Sequence DNA Replication Globins Molecular Sequence Data Oligodeoxyribonucleotides Oligonucleotides, Antisense RNA, Messenger Rabbits Reverse Transcriptase Inhibitors Templates, Genetic Journal Article

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N Loreau, C Boiziau, P Verspieren, D Shire, J J Toulmé. 1990-11-12. Blockage of AMV reverse transcriptase by antisense oligodeoxynucleotides.. https://doi.org/10.1016/0014-5793(90)81327-k

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