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PubMed · 17041268

Ribosome-inactivation display system.

Satoshi Fujita·Jing-Min Zhou·Kazunari Taira
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Abstract

We present a novel strategy for the connection of phenotype and genotype in vitro that can be used for the selection of functional proteins. The strategy involves the generation of a stable complex among a ribosome, an messenger RNA and its translated protein, without removal of the termination codon, as a result of the action of the ricin A chain during translation. The technique requires no transfection, no chemical synthesis, no ligation, and no removal of the termination codon. Thus, our novel ribosome-inactivation display system should provide, without loss of the pool population, a reliable, simple, and robust selection system for the in vitro evolution of the properties of proteins in a predictable direction by a combination of randomization and appropriate selection strategies.

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Amino Acid SequenceBase SequenceCodon, TerminatorDirected Molecular EvolutionGlutathione TransferaseModels, BiologicalModels, GeneticMolecular Sequence DataProtein BiosynthesisProtein EngineeringRNA, MessengerRibosomesRicinStreptavidinJournal ArticleResearch Support, Non-U.S. Gov't

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Satoshi Fujita, Jing-Min Zhou, Kazunari Taira. 2007. Ribosome-inactivation display system.. https://doi.org/10.1385/1-59745-187-8%3A221

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