Retrograde neuronal labelling by E. coli enterotoxin subunit B.

N Okado; H Hayashi; Y Hosoya; M Matsukawa

doi:10.1016/0304-3940(90)90055-e

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PubMed · 1705684

Retrograde neuronal labelling by E. coli enterotoxin subunit B.

N Okado·H Hayashi·Y Hosoya·M Matsukawa

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Abstract

The present communication reports that subunit B of Escherichia coli heat-labile enterotoxin (LTB) can be utilized as a powerful tracer in neuroanatomical studies. LTB was injected into the limb muscle of the chick, or into the superior cervical ganglion of the rat. Sections were processed with an immunohistochemical technique using an antibody against LTB. After the LTB injection into the muscle, retrogradely labelled motoneurons were found: the entire extent of dendritic arborizations appeared labeled. After the LTB injection into the ganglion, virtually all of the preganglionic neurons were retrogradely labelled in the rat spinal cord.

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Afferent Pathways Animals Axonal Transport Axons Bacterial Toxins Chickens Enterotoxins Escherichia coli Escherichia coli Proteins Ganglia, Sympathetic Muscles Neurons Rats Spinal Cord Journal Article Research Support, Non-U.S. Gov't

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N Okado, H Hayashi, Y Hosoya, M Matsukawa. 1990-12-11. Retrograde neuronal labelling by E. coli enterotoxin subunit B.. https://doi.org/10.1016/0304-3940(90)90055-e

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