PubMed · 17061854
A genetically encoded infrared probe.
Abstract
An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved that makes it possible to selectively and efficiently incorporate para-cyanophenylalanine (pCNPhe) into proteins in E. coli at sites specified by the amber nonsense codon, TAG. Substitution of pCNPhe for histidine-64 in myoglobin (Mb) affords a sensitive vibrational probe of ligand binding. This methodology provides a useful infrared reporter of protein structure, biomolecular interactions, and conformational changes.
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Kathryn C Schultz, Lubica Supekova, Youngha Ryu, Jianming Xie, Roshan Perera, Peter G Schultz. 2006-11-01. A genetically encoded infrared probe.. https://doi.org/10.1021/ja0636690
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