Diverse class 2 integrons in bacteria from beef cattle sources.

Robert S Barlow; Kari S Gobius

doi:10.1093/jac/dkl423

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PubMed · 17065187

Diverse class 2 integrons in bacteria from beef cattle sources.

Robert S Barlow·Kari S Gobius

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Abstract

OBJECTIVES: The purpose of this study was to determine the diversity of class 2 integrons in bacteria isolated from beef cattle sources. METHODS: The variable regions of a subset of 11 class 2 integron-containing bacteria were analysed by PCR and DNA sequencing for the presence of novel rearrangements. RESULTS: A total of six different class 2 integron arrays were identified and four of these were fully characterized. Three of the four arrays characterized have been previously described; however the remaining array is unlike previously described class 2 integrons. The novel class 2 integron was found in Providencia stuartii and contains an apparently functional class 2 integrase. Examination of the variable region of the P. stuartii integron identified nine open reading frames, mostly of unknown function, and represents the first report of a class 2 integron without inserted antibiotic resistance gene cassettes. CONCLUSIONS: This study has identified a novel class 2 integron found in P. stuartii that contains an apparently functional naturally occurring class 2 integrase. Further investigation of this novel class 2 integron is required to determine the impact of a functional class 2 integrase upon the evolution of class 2 integrons.

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Animals Bacteria Cattle DNA, Bacterial Drug Resistance, Bacterial Feces Gene Order Genetic Variation Genome, Bacterial Integrons Meat Molecular Sequence Data Open Reading Frames Polymerase Chain Reaction Providencia Recombination, Genetic Sequence Analysis, DNA Journal Article Research Support, Non-U.S. Gov't

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Robert S Barlow, Kari S Gobius. 2006-10-25. Diverse class 2 integrons in bacteria from beef cattle sources.. https://doi.org/10.1093/jac%2Fdkl423

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