PubMed · 1706608
Expression, purification, and crystallization of the HIV-1 reverse transcriptase (RT).
Abstract
The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.
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T Unge, H Ahola, R Bhikhabhai, K Bäckbro, S Lövgren, E M Fenyö, A Honigman, A Panet, J S Gronowitz, B Strandberg. 1990. Expression, purification, and crystallization of the HIV-1 reverse transcriptase (RT).. https://doi.org/10.1089/aid.1990.6.1297
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