Targeting vector construction by yeast artificial chromosome modification.

Peter J Murray

doi:10.1385/1-59745-158-4:127

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Targeting vector construction by yeast artificial chromosome modification.

Peter J Murray

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Abstract

Mouse yeast artificial chromosomes (YACs) are useful platforms for manipulation of targeting vector design and construction, particularly in circumstances where polymerase chain reaction-mediated amplification of targeting arms proves fruitless or the cloned DNA is inherently unstable. This chapter describes a simple procedure where YACs can be modified to produce targeting vectors of various sizes.

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Animals Chromosomes, Artificial, Yeast Cloning, Molecular Escherichia coli Female Gene Library Gene Targeting Genetic Vectors Mice Mice, Inbred C57BL Saccharomyces cerevisiae Stem Cells Journal Article

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Peter J Murray. 2006. Targeting vector construction by yeast artificial chromosome modification.. https://doi.org/10.1385/1-59745-158-4%3A127

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