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PubMed · 17074017

Epithelial microchimerism: consistent finding in human liver transplants.

Silvia Gaia·Susanna Cappia·Antonina Smedile·Elisa Bacillo·Ezio Gaia·Luciano Gubetta·Mario Rizzetto
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Abstract

BACKGROUND: Eleven liver biopsies from six male patients who received a liver transplant (LT) from female donors were examined in order to determine whether male host-derived hepatic cells were present in female grafts that exhibited minimal or important inflammatory damage. METHODS: Immunohistochemistry for epithelial cell type differentiation (anticytokeratin monoclonal antibody) and fluorescence in situ hybridization for XY chromosomes identification were performed on each slide. RESULTS: Host-derived hepatic cells were found in all except one transplant, with a frequency ranging from 2.3 to 25 per thousand of the total hepatocytes in the biopsy specimen. They were usually found as isolated cells scattered throughout the hepatic lobule; in one patient they were grouped into little clusters. Host-derived hepatic cells persisted throughout the histological follow up (up to 535 days after LT). Polyploidy for XY chromosome was observed. CONCLUSION: Hepatocytes derived from extra-hepatic stem cells are frequently found in small numbers in human liver grafts and persist over time.

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AgedBiopsyCell DifferentiationChimerismChromosomes, Human, XChromosomes, Human, YEpithelial CellsFemaleFollow-Up StudiesHepatocytesHumansImmunohistochemistryIn Situ Hybridization, FluorescenceLiver TransplantationMaleMiddle AgedPolyploidyRetrospective StudiesJournal ArticleResearch Support, Non-U.S. Gov't

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Silvia Gaia, Susanna Cappia, Antonina Smedile, Elisa Bacillo, Ezio Gaia, Luciano Gubetta, Mario Rizzetto. 2006. Epithelial microchimerism: consistent finding in human liver transplants.. https://doi.org/10.1111/j.1440-1746.2006.04675.x

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