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PubMed · 17081047

Comparison of hydroxylated print additives on antibody microarray performance.

Peng Wu·David W Grainger
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Abstract

Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, maintained up to 1 month at 4 degrees C for capturing model analytes, anti-human IL-1beta, IL-4, and TNFalpha, on these microarraying slides.

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AntibodiesBiotinylationHumansHydroxylationImage Processing, Computer-AssistedInterleukin-1betaInterleukin-4Oligonucleotide Array Sequence AnalysisProtein ConformationTumor Necrosis Factor-alphaJournal ArticleResearch Support, N.I.H., Extramural

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Peng Wu, David W Grainger. 2006. Comparison of hydroxylated print additives on antibody microarray performance.. https://doi.org/10.1021/pr060217d

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