A PCR-based method for detection and quantification of small RNAs.

Seungil Ro; Chanjae Park; Jingling Jin; Kenton M Sanders; Wei Yan

doi:10.1016/j.bbrc.2006.10.105

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A PCR-based method for detection and quantification of small RNAs.

Seungil Ro·Chanjae Park·Jingling Jin·Kenton M Sanders·Wei Yan

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Abstract

Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method.

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Animals Base Sequence Gene Expression Profiling Mice MicroRNAs Molecular Sequence Data Organ Specificity Polymerase Chain Reaction Tissue Distribution Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

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Seungil Ro, Chanjae Park, Jingling Jin, Kenton M Sanders, Wei Yan. 2006-10-30. A PCR-based method for detection and quantification of small RNAs.. https://doi.org/10.1016/j.bbrc.2006.10.105

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