[Methodological requirements for precise measurements using DNA single cell cytometry].

Abstract

Methodological aspects on DNA single cell cytometry were investigated. Specimens can be homogeneously Feulgen-stained if a high constancy of temperature is realized in the staining cuvette during acid hydrolysis. For the TV image analysis system under investigation (TAS-plus, Leitz) a linear correlation (r greater than 0.99) between physically defined optical transmission values and resulting electronic signals was found. Shading effects can be controlled by using a narrow mask width (VQ less than 1.5%). As an internal standard blood cells or epithelial cells can be used if a correction factor, that was found to be mainly constant, is calculated.