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Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing.

Markus Rueckel·Julia A Mack-Bucher·Winfried Denk
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Abstract

The image quality of a two-photon microscope is often degraded by wavefront aberrations induced by the specimen. We demonstrate here that resolution and signal size in two-photon microcopy can be substantially improved, even in living biological specimens, by adaptive wavefront correction based on sensing the wavefront of coherence-gated backscattered light (coherence-gated wavefront sensing, CGWS) and wavefront control by a deformable mirror. A nearly diffraction-limited focus can be restored even for strong aberrations. CGWS-based wavefront correction should be applicable to samples with a wide range of scattering properties and it should be possible to perform real-time pixel-by-pixel correction even at fast scan speeds.

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AnimalsGenes, ReporterLarvaMicroscopy, Fluorescence, MultiphotonZebrafishJournal ArticleResearch Support, Non-U.S. Gov't

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Markus Rueckel, Julia A Mack-Bucher, Winfried Denk. 2006-11-06. Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing.. https://doi.org/10.1073/pnas.0604791103

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