Cyclic AMP elicits biphasic current whose activation is mediated through protein phosphorylation in snail neurons.

K Watanabe; K Funase

doi:10.1016/0168-0102(91)90020-y

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Cyclic AMP elicits biphasic current whose activation is mediated through protein phosphorylation in snail neurons.

K Watanabe·K Funase

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Abstract

The involvement of protein phosphorylation in cAMP-induced transmembrane current was tested electrophysiologically and pharmacologically in identified neurons of the Japanese land snail, Euhadra peliomphala. Intracellular injection of cAMP elicited a biphasic transmembrane current (cAMP current) which consisted of inward and outward components. The inward component was blocked with Na(+)-free, Ca2(+)-free saline and the outward component abolished by either application of tetraethylammonium or a long-lasting exposure to caffeine in Ca2(+)-free saline. The cAMP current was completely suppressed by the protein kinase inhibitors, protein kinase inhibitor isolated from rabbit muscle or isoquinoline sulfonamide (H-8). The catalytic subunit of cAMP-dependent protein kinase transiently restored the cAMP current suppressed by H-8 nearly to pre-H-8 level. These findings suggest that protein phosphorylation may be an intermediate step in the activation process of the cAMP current.

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Animals Calcium Cyclic AMP Electrophysiology Ion Channels Isoquinolines Membrane Potentials Nerve Tissue Proteins Neurons Phosphorylation Protein Kinase C Protein Kinase Inhibitors Snails Sodium Journal Article Research Support, Non-U.S. Gov't

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K Watanabe, K Funase. 1991. Cyclic AMP elicits biphasic current whose activation is mediated through protein phosphorylation in snail neurons.. https://doi.org/10.1016/0168-0102(91)90020-y

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