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PubMed · 17115035

Sensitive and specific method for detecting G protein-coupled receptor mRNAs.

Arne Hansen·Yidong Chen·Jason M Inman·Quang N Phan·Zhi-Qing Qi·Charlie C Xiang·Miklos Palkovits·Natasha Cherman·Sergei A Kuznetsov·Pamela G Robey·Eva Mezey·Michael J Brownstein
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Abstract

G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

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Cell LineHumansOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionRNA, MessengerReceptors, G-Protein-CoupledSensitivity and SpecificityJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, N.I.H., IntramuralResearch Support, Non-U.S. Gov't

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Arne Hansen, Yidong Chen, Jason M Inman, Quang N Phan, Zhi-Qing Qi, Charlie C Xiang, Miklos Palkovits, Natasha Cherman, Sergei A Kuznetsov, Pamela G Robey, Eva Mezey, Michael J Brownstein. 2006-11-19. Sensitive and specific method for detecting G protein-coupled receptor mRNAs.. https://doi.org/10.1038/nmeth977

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