Probing molecular recognition sites on biosurfaces using AFM.

Vincent Dupres; Claire Verbelen; Yves F Dufrêne

doi:10.1016/j.biomaterials.2006.11.011

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Probing molecular recognition sites on biosurfaces using AFM.

Vincent Dupres·Claire Verbelen·Yves F Dufrêne

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Abstract

Knowledge of the molecular forces that drive receptor-ligand interactions is a key to gain a detailed understanding of cell adhesion events and to develop novel applications in biomaterials science. Until recently, there was no tool available for analyzing and mapping these forces on complex biosurfaces like cell surfaces. During the past decade, however, single-molecule atomic force microscopy (AFM) has opened exciting new opportunities for detecting and localizing molecular recognition forces on artificial biosurfaces and on living cells. In this review, we describe the general principles of the AFM technique, present procedures commonly used to prepare samples and tips, and discuss a number of applications that are relevant to the field of biomaterials.

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Animals Binding Sites Biocompatible Materials Cell Adhesion DNA Humans Membrane Proteins Microscopy, Atomic Force Protein Binding Surface Properties Journal Article Research Support, Non-U.S. Gov't Review

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Vincent Dupres, Claire Verbelen, Yves F Dufrêne. 2006-11-28. Probing molecular recognition sites on biosurfaces using AFM.. https://doi.org/10.1016/j.biomaterials.2006.11.011

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