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Differences in replication capacity between enterovirus 71 isolates obtained from patients with encephalitis and those obtained from patients with herpangina in Taiwan.

Chien-Min Kung·Chwan-Chuen King·Chun-Nan Lee·Li-Min Huang·Ping-Ing Lee·Chuan-Liang Kao
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Abstract

The cellular-tropism and biological characteristics of enterovirus 71 (EV71) isolates in Taiwan (TW) were studied. Growth curve experiments were conducted using cell lines that were possibly exhibited pathogenesis, and RT-PCR and sequencing tests were undertaken to amplify the 5' non-coding region (5'-NCR). The encephalitis isolate EV71 TW98NTU2078 was PBMC-tropic, temperature-resistant (Tr) at 40 degrees C, and easier to replicate in HTB-14 (astrocytoma) than the herpangina isolate EV71 TW98NTU1186 (The viral yields were 100-fold higher than those of the herpangina isolate EV71 TW98NTU1186 at 96 hr post infection.). The herpangina isolate EV71 TW98NTU1186 was non-PBMC-tropic, and temperature-sensitive (Ts) at 40 degrees C. The replication of EV71 TW98NTU1186 in HTB-14 was lower. No EV71 isolate infected HTB-37 (human colon adenocarcinoma cells). The encephalitis EV71 isolate exhibited better replication and transmission in PBMCs and astrocytes than did the EV71 isolate without CNS involvement.

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AnimalsChlorocebus aethiopsEncephalitis, ViralEnterovirusEnterovirus InfectionsHerpanginaHumansTaiwanVero CellsVirus ReplicationJournal ArticleResearch Support, Non-U.S. Gov't

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Chien-Min Kung, Chwan-Chuen King, Chun-Nan Lee, Li-Min Huang, Ping-Ing Lee, Chuan-Liang Kao. 2007. Differences in replication capacity between enterovirus 71 isolates obtained from patients with encephalitis and those obtained from patients with herpangina in Taiwan.. https://doi.org/10.1002/jmv.20761

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