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PubMed · 17141032

Embryonic stem cells from single blastomeres.

Sandy Becker·Young Chung
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Abstract

The fact that deriving embryonic stem (ES) cells from a blastocyst prevents its further development as an embryo is a major issue in human ES cell research. Using eight-cell mouse embryos, we have developed a method of deriving ES cells from a single blastomere, allowing the other seven to continue normal embryonic development. We remove one blastomere and coculture it with green fluorescent protein-labeled ES cells so that it is possible later to separate the clump of blastomere-derived ES cells to a feeder layer for further culturing. The removal of one blastomere is already performed on some human embryos during in vitro fertilization as part of prenatal genetic diagnosis.

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AnimalsBlastomeresCell DivisionCell LineCell SeparationDNA PrimersEmbryonic Stem CellsGenes, ReporterHumansMiceReverse Transcriptase Polymerase Chain ReactionJournal Article

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BibTeXRIS

Sandy Becker, Young Chung. 2006. Embryonic stem cells from single blastomeres.. https://doi.org/10.1016/s0076-6879(06)18007-6

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