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Surface-modified LPD nanoparticles for tumor targeting.

Shyh-Dar Li·Leaf Huang
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Abstract

We have developed a tumor-targeted LPD formulation (liposome-polycation-DNA complex) for siRNA. With surface modification, the targeted, PEGylated LPD increased the delivery efficiency by four-fold and the gene-silencing effect by two- to three-fold. Downregulation of survivin in human lung cancer cells by targeted LPD induced 90% of apoptosis and sensitized the cells to cisplatin by four-fold. PEGylated LPD formulation also significantly improved the tumor localization of siRNA in the NCI-H460 human lung cancer xenograft model. The tumor appeared to be the major uptake organ for siRNA formulated in surface-modified LPD. Our encouraging results indicate that surface-modified LPD may be a potent carrier for RNAi-based tumor therapy.

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AnimalsApoptosisBiomarkersCell Line, TumorCisplatinDown-RegulationDrug CarriersDrug SynergismGene SilencingGenetic TherapyHumansInhibitor of Apoptosis ProteinsLiposomesLung NeoplasmsMiceMice, NudeMicrotubule-Associated ProteinsNanoparticlesNeoplasm ProteinsPolyethylene GlycolsRNA, Small InterferingSurvivinTissue DistributionTransplantation, HeterologousJournal ArticleResearch Support, Non-U.S. Gov't

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Shyh-Dar Li, Leaf Huang. 2006. Surface-modified LPD nanoparticles for tumor targeting.. https://doi.org/10.1196/annals.1348.001

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