A ligase ribozyme obtained from a structured pool.

Wataru Yoshioka; Yoshiya Ikawa; Luc Jaeger; Tan Inoue

doi:10.1093/nass/48.1.209

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A ligase ribozyme obtained from a structured pool.

Wataru Yoshioka·Yoshiya Ikawa·Luc Jaeger·Tan Inoue

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Abstract

Here we report the in vitro selection experiment employed to construct a catalytic site on a self-folding RNA. To practice the selection, the sequence of 30 nucleotides was located in close proximity of the putative reaction site in a derivative of a naturally occurring RNA with a well known 3D structure. The acquired catalytic RNA retained the starting 3D structure and was successfully reconstructed according to its modular structure.

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Animals Base Sequence Ligases Molecular Sequence Data Nucleic Acid Conformation RNA, Catalytic Substrate Specificity Tetrahymena Journal Article

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Wataru Yoshioka, Yoshiya Ikawa, Luc Jaeger, Tan Inoue. 2004. A ligase ribozyme obtained from a structured pool.. https://doi.org/10.1093/nass%2F48.1.209

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