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PubMed · 17150639

Gene manipulation using artificial restriction DNA cutter.

Yoji Yamamoto·Jing-Min Zhou·Takafumi Tomita·Akihiko Uehara·Yuichiro Aiba·Akira Watanabe·Hiroyuki Aburatani·Makoto Komiyama
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Abstract

Artificial restriction DNA cutter (ARCUT), which we developed recently, was used for manipulation of plasmid DNA. PCR product was inserted into pBR322 plasmid vector using ARCUT, and E. coli cells were transfected with this recombinant plasmid DNA. Successful growth of cells shows that the recombination proceeds without any unexpected mutations. Furthermore, this artificial system was applied to PCR-free construction of chimera protein.

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CeriumDNAEscherichia coliGenetic VectorsHydrolysisPeptide Nucleic AcidsPlasmidsPolymerase Chain ReactionProtein EngineeringRecombinant Fusion ProteinsRecombination, GeneticTransfectionJournal ArticleResearch Support, Non-U.S. Gov't

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Yoji Yamamoto, Jing-Min Zhou, Takafumi Tomita, Akihiko Uehara, Yuichiro Aiba, Akira Watanabe, Hiroyuki Aburatani, Makoto Komiyama. 2005. Gene manipulation using artificial restriction DNA cutter.. https://doi.org/10.1093/nass%2F49.1.73

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