RNA isolation using immobilized PNA.

Takashi Ohtsuki; Chisato Kumano; Taishi Manabe; Mizuki Kitamatsu; Masahiko Sisido

doi:10.1093/nass/49.1.263

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PubMed · 17150734

RNA isolation using immobilized PNA.

Takashi Ohtsuki·Chisato Kumano·Taishi Manabe·Mizuki Kitamatsu·Masahiko Sisido

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Abstract

A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.

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Base Sequence Biotinylation Escherichia coli Molecular Sequence Data Peptide Nucleic Acids RNA, Bacterial RNA, Transfer RNA, Transfer, Leu Journal Article

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Takashi Ohtsuki, Chisato Kumano, Taishi Manabe, Mizuki Kitamatsu, Masahiko Sisido. 2005. RNA isolation using immobilized PNA.. https://doi.org/10.1093/nass%2F49.1.263

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