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PubMed · 17150738

Highly sensitive genotyping using artificial riboregulator system.

Atsushi Narita·Kazumasa Ogawa·Shinsuke Sando·Yasuhiro Aoyama
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Abstract

A molecular-beacon-type riboregulator (mRNA) was applied to multiply catalytic gene sensing. It consists of a reporter gene for firefly protein luciferase and, upstream thereof, a regulator hairpin domain composed of an RBS/anti-RBS stem (RBS = ribosome binding site) and a loop which is complementary to the target. The sensing of target gene, using an unmodified RNA or even dsDNA as a probe with a chemiluminescence output, was demonstrated with a sensitivity at < or = 50 fmol of the target and a single nucleotide resolution.

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Bacterial ProteinsGenes, ReporterGenotypeLuciferases, FireflyLuminescent AgentsLuminescent MeasurementsOligonucleotide ProbesProtein BiosynthesisRNA ProbesRNA, Small UntranslatedRNA, UntranslatedReceptors, CCR5Sequence Analysis, DNASigma FactorJournal ArticleResearch Support, Non-U.S. Gov't

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Atsushi Narita, Kazumasa Ogawa, Shinsuke Sando, Yasuhiro Aoyama. 2005. Highly sensitive genotyping using artificial riboregulator system.. https://doi.org/10.1093/nass%2F49.1.271

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