HIV gene therapy using RNA virus systems.

Masaaki Hayafune; Naoko Miyano-Kurosaki; Akiko Kusunoki; Yuka Mouri; Hiroshi Takaku

doi:10.1093/nass/nrl039

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PubMed · 17150826

HIV gene therapy using RNA virus systems.

Masaaki Hayafune·Naoko Miyano-Kurosaki·Akiko Kusunoki·Yuka Mouri·Hiroshi Takaku

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Abstract

We designed a vector to produce single-stranded DNA (ssDNA). We used HIV-1 reverse transcription for the purpose of constructing a DNAzyme expression vector against the HIV-1 env V3 loop. Initiation of HIV-1 reverse transcription requires the formation of a complex containing the viral RNA, tRNALys and reverse transcriptase. The expression vector contains the HIV-1 primer binding site (PBS) and tRNALys at the 3' end of its RNA transcript, thus enabling to an ssDNA would be synthesized by HIV-1 reverse transcriptase. We have demonstrated that the DNAzyme expressed by the lentiviral vectors suppressed HIV-1 replication in SupT1 cells.

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Cell Line DNA, Catalytic DNA, Single-Stranded Genetic Therapy Genetic Vectors HIV Infections HIV-1 Humans Lentivirus RNA, Viral Reverse Transcription Virus Replication Journal Article Research Support, Non-U.S. Gov't

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Masaaki Hayafune, Naoko Miyano-Kurosaki, Akiko Kusunoki, Yuka Mouri, Hiroshi Takaku. 2006. HIV gene therapy using RNA virus systems.. https://doi.org/10.1093/nass%2Fnrl039

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