PubMed Health⌕ Search

PubMed · 17152218

[Abiotrophia and Granulicatella].

Abstract

NVS grow in culture media with 0.001% pyridoxal hydrochloride (PHC) and 0.1% cysteine hydrochloride (CysHC). These bacteria need the help of other organisms to grow on common solid media showing the effect known as "satellitism". Both, satellitism and the pyrrolidonilarilamidase test are the key tests for suspecting the presence of NVS. They can grow as a faint haze on blood agar or chocolate agar prepared with the Columbia agar base without adding any other substance. The most frequently documented infections are endocarditis. Among extravascular infections, ocular infections predominate. For antimicrobial susceptibility testing, the Etest and dilution methods with the addition of 0.001% PHC can be used. Whichever the method there does not seem to be much correlation in vitro/ in vivo. The rate of penicillin resistance and the MICs were similar to those observed in viridans group streptococci. Four to six weeks of penicillin plus gentamicin is recommended for the treatment of VNS endocarditis. In cases of treatment failure or beta-lactam allergic susceptibility, vancomycin alone or with the addition of gentamicin and/or rifampin is used.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Horacio A Lopardo. [Abiotrophia and Granulicatella].. https://pubmed.ncbi.nlm.nih.gov/17152218/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

In vitro studies with DQ-113 and comparison fluoroquinolones to determine propensities to select resistance in gram-positive cocci.

DQ-113 was compared in vitro to sitafloxacin, moxifloxacin, levofloxacin, and ciprofloxacin for potential to select mutational resistance in multiresistant staphylococci, pneumococci, and enterococci. Its ability to select less-susceptible mutants varied according to species, being lowest with staphylococci, intermediate with pneumococci, and greatest with enterococci.

Drug Resistance, Bacterial↗

Evolution of TEM-type enzymes: biochemical and genetic characterization of two new complex mutant TEM enzymes, TEM-151 and TEM-152, from a single patient.

Two clinical isolates of Escherichia coli, CF1179 and CF1295, were isolated from a patient hospitalized in the hematology unit of the University Hospital of Clermont-Ferrand, Clermont-Ferrand, France. They were resistant to penicillin-clavulanate combinations and to ceftazidime. The double-disk synergy test was positive only for isolate CF1179. Molecular comparison of the isolates showed that they were clonally related. E. coli recombinant strains exhibiting the resistance phenotype of the clinical strains were obtained by cloning. The clones corresponding to strains CF1179 and CF1295 produced TEM-type beta-lactamases with pI values of 5.7 and 5.3, respectively. Sequencing analysis revealed two novel blaTEM genes encoding closely related complex mutant TEM enzymes, designated TEM-151 (pI 5.3) and TEM-152 (pI 5.7). These two genes also harbored a new promoter region which presented a 9-bp deletion. The two novel beta-lactamases differed from the parental enzyme, TEM-1, by the substitution Arg164His, previously observed in extended-spectrum beta-lactamases (ESBLs), and by the substitutions Met69Val and Asn276Asp, previously observed in the inhibitor-resistant penicillinase TEM-36/IRT-7. They differed by two amino acid substitutions: TEM-152 harbored a Glu240Lys ESBL-type substitution and TEM-151 had an Ala284Gly substitution. Functional analysis of TEM-151 and TEM-152 showed that both enzymes had hydrolytic activity against ceftazidime (kcat, 5 and 16 s-1, respectively). TEM-152 was more resistant than TEM-151 to the inhibitor clavulanic acid (50% inhibitory concentrations, 1 versus 0.17 microM). These results confirm the evolution of TEM-type enzymes toward complex enzymes harboring the two kinds of substitutions which confer an extended spectrum of action against beta-lactam antibiotics and resistance to inhibitors.

Drug Resistance, Bacterial↗

[Infection control strategies for antimicrobial-resistance].

Antimicrobial-resistance (AMR) is a growing threat especially to critically ill patients, because treatment failure can have serious consequences. MRSA has been a major concern in Japanese hospitals, however, emerging the drug-resistant pathogens that comprise VRE, MDRP or ESBL have been reported as nosocomial infectious pathogens. To control antimicrobial-resistance, antimicrobial therapy according to local sensitivity result and multidisciplinary management is necessary. CDC recommends 12 steps to prevent AMR among hospitalized patients, which included prevent infection, diagnose and treat infection effectively, use antimicrobials wisely, and prevent transmission. Infectious diseases experts, who are far lacking in Japan, may play a key role to achieve these recommendations.

Drug Resistance, Bacterial↗