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Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis.

Donglin Liu·J Michael Brockman·Brinda Dass·Lucie N Hutchins·Priyam Singh·John R McCarrey·Clinton C MacDonald·Joel H Graber
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Abstract

Gene expression and processing during mouse male germ cell maturation (spermatogenesis) is highly specialized. Previous reports have suggested that there is a high incidence of alternative 3'-processing in male germ cell mRNAs, including reduced usage of the canonical polyadenylation signal, AAUAAA. We used EST libraries generated from mouse testicular cells to identify 3'-processing sites used at various stages of spermatogenesis (spermatogonia, spermatocytes and round spermatids) and testicular somatic Sertoli cells. We assessed differences in 3'-processing characteristics in the testicular samples, compared to control sets of widely used 3'-processing sites. Using a new method for comparison of degenerate regulatory elements between sequence samples, we identified significant changes in the use of putative 3'-processing regulatory sequence elements in all spermatogenic cell types. In addition, we observed a trend towards truncated 3'-untranslated regions (3'-UTRs), with the most significant differences apparent in round spermatids. In contrast, Sertoli cells displayed a much smaller trend towards 3'-UTR truncation and no significant difference in 3'-processing regulatory sequences. Finally, we identified a number of genes encoding mRNAs that were specifically subject to alternative 3'-processing during meiosis and postmeiotic development. Our results highlight developmental differences in polyadenylation site choice and in the elements that likely control them during spermatogenesis.

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3' Untranslated RegionsAnimalsEvolution, MolecularExpressed Sequence TagsMaleMicePolyadenylationRNA 3' Polyadenylation SignalsRNA, MessengerSpermatogenesisTestismRNA Cleavage and Polyadenylation FactorsJournal ArticleResearch Support, N.I.H., Extramural

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Donglin Liu, J Michael Brockman, Brinda Dass, Lucie N Hutchins, Priyam Singh, John R McCarrey, Clinton C MacDonald, Joel H Graber. 2006-12-08. Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis.. https://doi.org/10.1093/nar%2Fgkl919

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