Cryptococcus neoformans produces authentic prostaglandin E2 without a cyclooxygenase.

John R Erb-Downward; Gary B Huffnagle

doi:10.1128/ec.00336-06

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PubMed · 17158733

Cryptococcus neoformans produces authentic prostaglandin E2 without a cyclooxygenase.

John R Erb-Downward·Gary B Huffnagle

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Abstract

Many single-celled eukaryotes produce prostaglandin-like molecules, but these have not been absolutely verified by mass spectrometry. We have isolated, and identified by liquid chromatography-tandem mass spectrometry, authentic prostaglandin E(2) from Cryptococcus neoformans. Cyclooxygenase inhibitors did not inhibit prostaglandin synthesis, and the cryptococcal genome lacks a cyclooxygenase homolog. Thus, novel enzymes must exist.

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Blotting, Western Cells, Cultured Chromatography, Liquid Cryptococcus neoformans Cyclooxygenase 1 Cyclooxygenase 2 Cyclooxygenase Inhibitors Dinoprostone Mass Spectrometry Journal Article Research Support, N.I.H., Extramural

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John R Erb-Downward, Gary B Huffnagle. 2006-12-08. Cryptococcus neoformans produces authentic prostaglandin E2 without a cyclooxygenase.. https://doi.org/10.1128/ec.00336-06

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