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PubMed · 1716637

Two-parameter mobile phase optimization for the simultaneous high-performance liquid chromatographic determination of dopamine, serotonin and related compounds in microdissected rat brain nuclei.

M Lema·J Otero·J Marcó
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Abstract

A new high-pressure liquid chromatography method with electrochemical detection is described that allows the simultaneous determination of dopamine, serotonin, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylacetic acid, 5-hydroxytryptophan and 5-hydroxyindoleacetic acid in microdissected nuclei from individual rat brains. No sample pre-treatment steps are required. Resolution and analysis time were optimized by a simple limited optimization procedure, involving two-parameter factorial design.

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3,4-Dihydroxyphenylacetic Acid5-HydroxytryptophanAnimalsBrainCell NucleusChromatography, High Pressure LiquidDopamineHydroxyindoleacetic AcidMaleMethoxyhydroxyphenylglycolNorepinephrineRatsRats, Inbred StrainsSerotoninJournal ArticleResearch Support, Non-U.S. Gov't

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M Lema, J Otero, J Marcó. 1991-06-28. Two-parameter mobile phase optimization for the simultaneous high-performance liquid chromatographic determination of dopamine, serotonin and related compounds in microdissected rat brain nuclei.. https://doi.org/10.1016/s0021-9673(01)88634-9

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