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PubMed · 1716726

Molecular analysis of the Bacillus subtilis recF function.

J C Alonso·A C Stiege
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Abstract

recF resides between the dnaN and gyrB genes of Bacillus subtilis. The recF15 mutation results in replacement of a glutamate residue in the wild type with a lysine residue in the mutant RecF protein. We investigated the in vivo regulation of recF using a transcriptional fusion to the xylE gene and assaying mRNA production. We found that novobiocin leads to a four-fold induction in recF gene expression, but this is not observed in a gyrB mutant strain. Enhancement of expression of the recF gene in the presence of novobiocin is unrelated to the SOS response. The RecF protein, which has a predicted molecular mass of 42.2 kDa, does not seem to be involved in its own regulation.

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Bacillus subtilisDNA RepairGene ExpressionGenes, BacterialNovobiocinPlasmidsRNA, BacterialRNA, MessengerSOS Response, GeneticsTopoisomerase II InhibitorsTranscription, GeneticJournal Article

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J C Alonso, A C Stiege. 1991. Molecular analysis of the Bacillus subtilis recF function.. https://doi.org/10.1007/bf00260632

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