A pipeline for ligand discovery using small-molecule microarrays.

Jay L Duffner; Paul A Clemons; Angela N Koehler

doi:10.1016/j.cbpa.2006.11.031

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A pipeline for ligand discovery using small-molecule microarrays.

Jay L Duffner·Paul A Clemons·Angela N Koehler

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Abstract

Uncovering the functions of thousands of gene products, in various states of post-translational modification, is a key challenge in the post-genome era. To identify small-molecule probes for each protein function, high-throughput methods for ligand discovery are needed. In recent years, small-molecule microarrays (SMMs) have emerged as high-throughput and miniaturized screening tools for discovering protein-small-molecule interactions. Microarrays of small molecules from a variety of sources, including FDA-approved drugs, natural products and products of combinatorial chemistry and diversity-oriented synthesis, have been prepared and screened by several laboratories, leading to several newly discovered protein-ligand pairs.

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Biological Products Chemistry, Pharmaceutical Combinatorial Chemistry Techniques Ligands Microarray Analysis Molecular Probes Peptides Protein Processing, Post-Translational Sensitivity and Specificity Journal Article Research Support, N.I.H., Extramural Review

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Jay L Duffner, Paul A Clemons, Angela N Koehler. 2006-12-13. A pipeline for ligand discovery using small-molecule microarrays.. https://doi.org/10.1016/j.cbpa.2006.11.031

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