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PubMed · 17170255

Histocompatible embryonic stem cells by parthenogenesis.

Kitai Kim·Paul Lerou·Akiko Yabuuchi·Claudia Lengerke·Kitwa Ng·Jason West·Andrew Kirby·Mark J Daly·George Q Daley
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Abstract

Genetically matched pluripotent embryonic stem (ES) cells generated via nuclear transfer or parthenogenesis (pES cells) are a potential source of histocompatible cells and tissues for transplantation. After parthenogenetic activation of murine oocytes and interruption of meiosis I or II, we isolated and genotyped pES cells and characterized those that carried the full complement of major histocompatibility complex (MHC) antigens of the oocyte donor. Differentiated tissues from these pES cells engrafted in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation.

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AnimalsCell DifferentiationCell LineChromosome SegregationEmbryonic Stem CellsFemaleGenotypeH-2 AntigensHeterozygoteHistocompatibilityHistocompatibility Antigens Class IIMajor Histocompatibility ComplexMeiosisMiceMice, Inbred C57BLMice, Inbred CBAOocytesParthenogenesisPluripotent Stem CellsPolymerase Chain ReactionRecombination, GeneticStem Cell TransplantationJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't

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Kitai Kim, Paul Lerou, Akiko Yabuuchi, Claudia Lengerke, Kitwa Ng, Jason West, Andrew Kirby, Mark J Daly, George Q Daley. 2006-12-14. Histocompatible embryonic stem cells by parthenogenesis.. https://doi.org/10.1126/science.1133542

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