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Focal plane array infrared imaging: a new way to analyse leaf tissue.

Philip Heraud·Sally Caine·Gordon Sanson·Ros Gleadow·Bayden R Wood·Don McNaughton
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Abstract

* Here, a new approach to macromolecular imaging of leaf tissue using a multichannel focal plane array (FPA) infrared detector was compared with the proven method of infrared mapping with a synchrotron source, using transverse sections of leaves from a species of Eucalyptus. * A new histological method was developed, ideally suited to infrared spectroscopic analysis of leaf tissue. Spatial resolution and the signal-to-noise ratio of the FPA imaging and synchrotron mapping methods were compared. * An area of tissue 350 microm(2) required approx. 8 h to map using the synchrotron technique and approx. 2 min to image using the FPA. The two methods produced similar infrared images, which differentiated all tissue types in the leaves according to their macromolecular chemistry. * The synchrotron and FPA methods produced similar results, with the synchrotron method having superior signal-to-noise ratio and potentially better spatial resolution, whereas the FPA method had the advantage in terms of data acquisition time, expense and ease of use. FPA imaging offers a convenient, laboratory-based approach to microscopic chemical imaging of leaves.

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EucalyptusInfrared RaysPlant LeavesSpectrophotometry, InfraredSynchrotronsComparative StudyEvaluation StudyJournal Article

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Philip Heraud, Sally Caine, Gordon Sanson, Ros Gleadow, Bayden R Wood, Don McNaughton. 2007. Focal plane array infrared imaging: a new way to analyse leaf tissue.. https://doi.org/10.1111/j.1469-8137.2006.01881.x

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