PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 17177249

Biospecific interaction (affinity) CEC and affinity nano-LC.

Fred M Okanda·Ziad El Rassi
View original source ↗Export metadata ↓

Abstract

This review article is aimed at assessing the recent progress made in affinity nano-LC and affinity CEC performed in capillaries and microchips. A variety of biospecific interactions is covered including lectin affinity, immunoaffinity, immobilized metal affinity, sugar-based affinity, protein A affinity, protein G affinity, aptamer affinity, enzyme affinity, and other miscellanea. ACE involving affinity interaction in free solution is not covered in this review article. Also, affinity-based separations involving chiral recognition are not the subject of this review article because they are the topic of a more specialized review article on chiral separations in this special issue. A total of 31 papers published in the period 1998-2006 have been discussed in this review article.

Explore related subjects

Capillary ElectrochromatographyChromatography, AffinityMicrofluidic Analytical TechniquesNanotechnologyProteinsJournal ArticleResearch Support, Non-U.S. Gov'tReview

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Fred M Okanda, Ziad El Rassi. 2007. Biospecific interaction (affinity) CEC and affinity nano-LC.. https://doi.org/10.1002/elps.200600595

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

Recent progress in polar stationary phases for CEC.

This review summarizes most of the recent developments in the preparation and application of polar stationary phases for CEC covering the literature published since the year 2004. These polar stationary phases have been adopted for separation of analytes by the modes of packing column CEC, open-tubular CEC (o-CEC) and monolithic column CEC. Currently, development of o-CEC using biomolecules, such as protein and DNA, as the immobilized ligands is highlighted partly due to the simplicity of preparation. Furthermore, monolithic columns have been extended quickly, particularly inorganic materials-based monoliths, such as silica, zirconia, hafnium, etc., as an alternative to packed columns have been developed quickly.

Capillary Electrochromatography↗
PubMed2007

Advances in chiral separation using capillary electromigration techniques.

This review gives an overview of recent developments in CZE, EKC, and CEC covering the literature since the year 2004. Since there appeared a special issue on applications, this review focuses on the progress in electromigration techniques and new methodological developments. New techniques, new chiral selectors as well as new chiral stationary phases for CEC are discussed.

Capillary Electrochromatography↗
PubMed2007

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips.

Poor sensitivity is considered to be one of the major limitations of electrophoretic separation methods, particularly when compared to traditional liquid chromatographic techniques. To address this issue, various in-line preconcentration techniques have been developed over the past 15 years, ranging in power and complexity, and there are now a number of well understood approaches routinely capable of providing a 10,000- to 100,000-fold increase in sensitivity, as well as several that can be pushed above a million. Furthermore, these have been achieved with particularly troublesome and often difficult samples, such as those having high salinity from a biological or environmental origin. This review will discuss the most common methods for improving the sensitivity of CE, CEC and microchip version of these, with particular attention to those approaches developed over the last five years.

Capillary Electrochromatography↗