SILENCE: a new forward genetic technology.

David J Banks; Kenneth A Bradley

doi:10.1038/nmeth991

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PubMed · 17179935

SILENCE: a new forward genetic technology.

David J Banks·Kenneth A Bradley

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Abstract

Silencing induced by long terminal repeat (LTR)-encoded cis-acting response element, termed SILENCE, is a forward genetic system that allows for conditional, epigenetic control of host-gene transcription. This new research tool is independent of gene mutation or disruption, does not require complementation, and conditional gene repression appears complete at the level of protein function. SILENCE functions in hypodiploid cells and is a platform technology with broad applications in gene discovery.

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Animals Antigens, Bacterial Bacterial Toxins CHO Cells Clone Cells Cricetinae Cricetulus Gene Silencing Genetic Techniques Mutation Phenotype Receptors, Peptide Structure-Activity Relationship Terminal Repeat Sequences Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

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David J Banks, Kenneth A Bradley. 2006-12-17. SILENCE: a new forward genetic technology.. https://doi.org/10.1038/nmeth991

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