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Expression and function of surface antigens on scleroderma fibroblasts.

D Abraham·S Lupoli·A McWhirter·C Plater-Zyberk·T H Piela·J H Korn·I Olsen·C Black
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Abstract

Dermal fibroblasts from patients with systemic sclerosis (SSc) bound a much greater number of T lymphocytes than did normal dermal fibroblasts. Monoclonal antibodies (MAb) against classes I and II antigens of the major histocompatibility complex (MHC) and their receptors, CD8 and CD4, had no effect on T cell interaction with SSc and normal cells, while MAb against lymphocyte function-associated antigen type 3 (LFA-3) and CD2 both strongly inhibited lymphocyte attachment. MAb against intercellular adhesion molecule type 1 (ICAM-1) and LFA-1 also prevented binding of T lymphocytes, but had a more marked effect on adhesion to SSc fibroblasts than to normal fibroblasts; they also completely abolished the increased binding to fibroblasts treated with interleukin-1 alpha, tumor necrosis factor alpha, and interferon-gamma. No difference was found in the proportion of normal and SSc fibroblasts that expressed MHC classes I and II and LFA-3, but more SSc cells expressed ICAM-1, and at a higher level, than did normal fibroblasts. These results show that cultured SSc cells have elevated binding to T lymphocytes, which possibly results from expansion of a subset of fibroblasts that produces high levels of ICAM-1.

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AdultAgedAntibodies, MonoclonalAntigens, Differentiation, T-LymphocyteAntigens, SurfaceCD2 AntigensCD58 AntigensCell AdhesionCell Adhesion MoleculesFemaleFibroblastsHumansIntercellular Adhesion Molecule-1Interferon-gammaInterleukin-1MaleMembrane GlycoproteinsMiddle AgedReceptors, ImmunologicScleroderma, SystemicT-LymphocytesTumor Necrosis Factor-alphaJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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D Abraham, S Lupoli, A McWhirter, C Plater-Zyberk, T H Piela, J H Korn, I Olsen, C Black. 1991. Expression and function of surface antigens on scleroderma fibroblasts.. https://doi.org/10.1002/art.1780340913

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