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PubMed · 17185779

Quantitative proteomics for two-dimensional gels using difference gel electrophoresis.

David B Friedman
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Abstract

Difference gel electrophoresis (DIGE) technology provides a powerful quantitative component to proteomics experiments involving two-dimensional (2D) gel electrophoresis. DIGE allows for the detection of subtle changes in protein abundance with statistical confidence while controlling for gel-to-gel variation, as well as additional variation that is non-biological in origin (e.g., sample preparation error, normal variation in a system). Samples are differentially labeled with spectrally resolvable fluorescent dyes (Cy2, Cy3, and Cy5) and co-resolved for direct quantification within the same 2D gel. Increased statistical confidence is obtained when combining experimental repetition with internal standards such that independent replicate measurements from single- and multivariable analyses can be intercompared with a relatively small number of coordinated DIGE gels.

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Electrophoresis, Gel, Two-DimensionalProteomicsSoftwareStaining and LabelingJournal Article

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David B Friedman. 2007. Quantitative proteomics for two-dimensional gels using difference gel electrophoresis.. https://doi.org/10.1385/1-59745-275-0%3A219

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