An Escherichia coli expression-based method for heme substitution.

Joshua J Woodward; Nathaniel I Martin; Michael A Marletta

doi:10.1038/nmeth984

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PubMed · 17187078

An Escherichia coli expression-based method for heme substitution.

Joshua J Woodward·Nathaniel I Martin·Michael A Marletta

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Abstract

Heme reconstitution with porphyrin analogs is a powerful approach toward understanding the molecular function of heme proteins; present methods, however, have not proven to be generally useful. Here we describe the development and application of an expression-based method for introducing modified porphyrins. The approach allows efficient incorporation of heme analogs using a widely available bacterial strain and offers an attractive alternative to present reconstitution methods that subject proteins to harsh, denaturing conditions.

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Chromatography, High Pressure Liquid Escherichia coli Hemeproteins Porphyrins Sensitivity and Specificity Spectrophotometry, Ultraviolet Time Factors Journal Article Research Support, Non-U.S. Gov't

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Joshua J Woodward, Nathaniel I Martin, Michael A Marletta. 2006-12-24. An Escherichia coli expression-based method for heme substitution.. https://doi.org/10.1038/nmeth984

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