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Microfluidic flow-flash: method for investigating protein dynamics.

Michael W Toepke·Scott H Brewer·Dung M Vu·Kirk D Rector·Joel E Morgan·Robert B Gennis·Paul J A Kenis·R Brian Dyer
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Abstract

We report a new method, microfluidic flow-flash, for measuring protein reaction kinetics. The method couples a microscope imaging detection system with a microfluidic flow cell to reduce data acquisition times and sample consumption. This combination allows for the simultaneous collection of spectral and temporal information. The microfluidic flow cell design utilizes three-dimensional sheath flow to reduce sample dispersion and minimize sample consumption. The ability to alter the flow rates in the microfluidic flow cells allows a variety of time scales to be studied with submillisecond time resolution. The imaging detection system can be coupled with several spectroscopic probes including fluorescence and UV/visible absorbance spectroscopy. Here, we utilize the microfluidic flow-flash method to probe the kinetics of CO recombination or O2 binding to myoglobin after the laser-induced photolysis of CO from myoglobin by UV/visible absorbance spectral imaging.

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Carbon MonoxideFluorescenceKineticsLasersMicrofluidicsMicroscopy, ConfocalMyoglobinOxygenPhotolysisSpectrometry, FluorescenceSpectrophotometry, UltravioletJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Michael W Toepke, Scott H Brewer, Dung M Vu, Kirk D Rector, Joel E Morgan, Robert B Gennis, Paul J A Kenis, R Brian Dyer. 2007-01-01. Microfluidic flow-flash: method for investigating protein dynamics.. https://doi.org/10.1021/ac061193x

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