Toxin binding to chimeric K+ channels immobilised on a solid nitrocellulose support.

Christian Legros; Marie-France Martin-Eauclaire; Olaf Pongs; Pierre E Bougis

doi:10.1016/j.bbrc.2006.12.156

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Toxin binding to chimeric K+ channels immobilised on a solid nitrocellulose support.

Christian Legros·Marie-France Martin-Eauclaire·Olaf Pongs·Pierre E Bougis

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Abstract

In this work, we used a panel prokaryote/eukaryote K+ channel chimeras to generate K+ channel arrays. Their behaviour in solution was compared with that when spotted on a nitrocellulose-supported film and their responses to selective high affinity ligands, including polypeptide toxins and TEA, were studied.

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Animals Bacterial Proteins Binding, Competitive Charybdotoxin Collodion Dose-Response Relationship, Drug Humans Iodine Radioisotopes Kinetics Kv1.3 Potassium Channel Microarray Analysis Potassium Channels Recombinant Fusion Proteins Scorpion Venoms Tetraethylammonium Toxins, Biological Journal Article Research Support, Non-U.S. Gov't

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Christian Legros, Marie-France Martin-Eauclaire, Olaf Pongs, Pierre E Bougis. 2006-12-28. Toxin binding to chimeric K+ channels immobilised on a solid nitrocellulose support.. https://doi.org/10.1016/j.bbrc.2006.12.156

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