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PubMed · 17200809

A folic acid-based functionalized surface for biosensor systems.

Kaustubh D Bhalerao·Stephen C Lee·Winston O Soboyejo·Alfred B O Soboyejo
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Abstract

The performance of a biosensor depends largely on its interface with the biological system. This interface imparts a biologically relevant function to the device and provides a measure of specificity towards the biological analyte of interest. This paper documents the choice of folic acid as the functional component of a cantilever sensor to recognize nasopharyngeal (KB) cancer cells. A conjugation chemistry protocol has been outlined to deploy folic acid onto a titanium-coated sensor surface using a silane linker. The presence and biological activity of the sensor was verified by means of an immunospecific (ELISA) procedure. The overall performance of the folic acid-based cantilever sensor was measured using cancerous KB cell-binding experiments.

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Biosensing TechniquesCell Line, TumorCoated Materials, BiocompatibleEnzyme-Linked Immunosorbent AssayFolic AcidHumansMolecular StructureNasopharyngeal NeoplasmsPropylaminesReproducibility of ResultsSensitivity and SpecificitySilanesSurface PropertiesTitaniumJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Kaustubh D Bhalerao, Stephen C Lee, Winston O Soboyejo, Alfred B O Soboyejo. 2007. A folic acid-based functionalized surface for biosensor systems.. https://doi.org/10.1007/s10856-006-0657-x

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