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Altered brain lipid composition in cyclooxygenase-2 knockout mouse.

Kaizong Ma·Robert Langenbach·Stanley I Rapoport·Mireille Basselin
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Abstract

Cyclooxygenase (COX)-2 plays an important role in brain arachidonic acid (20:4n-6) metabolism, and its expression is upregulated in animal models of neuroinflammation and excitotoxicity. Our hypothesis was that brain lipid composition would be altered in COX-2 knockout (COX-2(-/-)) compared with wild-type (COX-2(+/+)) mice, reflecting the important role of COX-2 in brain lipid metabolism. Concentrations of different lipids were measured in high-energy microwaved brain from COX-2(-/-) and COX-2(+/+) mice. Compared with the COX-2(+/+) mouse brain, the brain of the COX-2(-/-) mouse had a statistically significant 15% increase in phosphatidylserine (PtdSer) and significant 37, 27, and 32% reductions in triacylglycerol and cholesterol concentrations and in the cholesterol-to-phospholipid ratio, respectively. The normalized concentration of palmitic acid (16:0) was increased in PtdSer, as was the brain concentration of unesterified arachidic acid (20:0). A lifetime absence of COX-2 produces multiple changes in brain lipid composition. These changes may be related to reported changes in fatty acid kinetics and in resistance to neuroinflammation and excitotoxicity in the COX-2(-/-) mouse.

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AnimalsBrain ChemistryCyclooxygenase 2Fatty AcidsLipid MetabolismLipidsMiceMice, KnockoutJournal ArticleResearch Support, N.I.H., Intramural

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Kaizong Ma, Robert Langenbach, Stanley I Rapoport, Mireille Basselin. 2007-01-03. Altered brain lipid composition in cyclooxygenase-2 knockout mouse.. https://doi.org/10.1194/jlr.m600400-jlr200

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