RNA interference in mice.

R Kühn; S Streif; W Wurst

doi:10.1007/978-3-540-35109-2_7

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PubMed · 17203655

RNA interference in mice.

R Kühn·S Streif·W Wurst

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Abstract

Silencing of gene expression by RNA interference (RNAi) has become a powerful tool for functional genomics in mammalian cells. Furthermore, RNAi holds promise as a simple, fast and cost-effective approach to studying mammalian gene function in vivo and as a novel therapeutic approach. This review provides an overview of the progress of RNAi in vivo, with emphasis on systemic/local siRNA delivery, viral shRNA vectors, shRNA vector transgenic mice and conditional systems to control shRNA vectors. Taken together, the data from 80 in vivo studies show that RNAi is a useful tool that offers new opportunities for functional genomics in mice.

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Animals Genetic Vectors Mice Mice, Transgenic RNA Interference RNA, Small Interfering RNA, Viral Journal Article Research Support, Non-U.S. Gov't Review

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R Kühn, S Streif, W Wurst. 2007. RNA interference in mice.. https://doi.org/10.1007/978-3-540-35109-2_7

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