Analysis of mouse development with conditional mutagenesis.

M Lewandoski

doi:10.1007/978-3-540-35109-2_10

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Analysis of mouse development with conditional mutagenesis.

M Lewandoski

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Abstract

Explorations into the molecular embryology of the mouse have played a vital role in our understanding of the basic mechanisms of gene regulation that govern development and disease. In the last 15 years, these mechanisms have been analyzed with vastly greater precision and clarity with the advent of systems that allow the conditional control of gene expression. Typically, this control is achieved by silencing or activating the gene of interest with site-specific DNA recombination or transcriptional transactivation. In this review, I discuss the application of these technologies to mouse development, focusing on recent innovations and experimental designs that specifically aid the study of the mouse embryo.

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Animals Gene Targeting Mice Mice, Transgenic Mutagenesis RNA Interference Recombinases Journal Article Research Support, N.I.H., Intramural Review

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M Lewandoski. 2007. Analysis of mouse development with conditional mutagenesis.. https://doi.org/10.1007/978-3-540-35109-2_10

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