[An efficient mutational method for photosynthetic bacteria].

J Q Lin; M Xiao; M T Long; B Han; W Quian; J Du

PubMed · 17205800

[An efficient mutational method for photosynthetic bacteria].

J Q Lin·M Xiao·M T Long·B Han·W Quian·J Du

Abstract

The pigment and auxotrophic mutants of Rhodobacter sphaeroides Y6 were obtained by treatment with ethyl methanesulfonate (EMS) followed by lithium chloride (LiCI). Treatment with 0.081 M EPS and subsequent treatment with 0.071 M LiCI resulted in 12% higher frequency of pigment mutations than application of 0.081 M EMS alone; the frequency of auxotrophic mutations increased 2.5-fold when treatment with lithium chloride was applied. A blue shift 10 nm was recorded in the absorption spectrum of carotenoids form YM5-3 green mutant; considerable accumulation of neurosporine was revealed by HPLC and mass spectrometry. The method is efficient for isolating mutants of photosynthetic bacteria.

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Ethyl Methanesulfonate Lithium Chloride Mutation Photosynthesis Pigments, Biological Rhodobacter sphaeroides English Abstract Journal Article Research Support, Non-U.S. Gov't

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J Q Lin, M Xiao, M T Long, B Han, W Quian, J Du. [An efficient mutational method for photosynthetic bacteria].. https://pubmed.ncbi.nlm.nih.gov/17205800/

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