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PubMed · 17208018

A comprehensive DNA sequence library is essential for identification with DNA barcodes.

Torbjørn Ekrem·Endre Willassen·Elisabeth Stur
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Abstract

In this study we examine the possibility of utilising partial cox1 gene sequences as barcodes to identify non-biting midges (Diptera: Chironomidae). We analysed DNA from 97 specimens of 47 species in the genera Cladotanytarsus, Micropsectra, Parapsectra, Paratanytarsus, Rheotanytarsus, Tanytarsus and Virgatanytarsus with a main focus on Micropsectra, Parapsectra and Paratanytarsus. Our findings show that (1) cox1 is easily amplified from extracts from different life stages with the standard barcoding primers. (2) Although K2P-distances between con-specific sequences varied up to 4.9%, con-specifics clustered together with 91-100% bootstrap support in maximum parsimony analysis. This indicates that barcodes may be excellent tools to identify species that are already in a cox1 library. (3) Both neighbour joining and maximum parsimony failed to reconstruct monophyletic genera. Thus, if a well-matching cox1 sequence is not already available in the library, the prospects of approximately identifying an unknown taxon, even to the correct genus of subtribe Tanytarsina, are not good.

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AnimalsChironomidaeDNAElectron Transport Complex IVFemaleGene LibraryGenes, InsectMalePhylogenyJournal ArticleResearch Support, Non-U.S. Gov't

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Torbjørn Ekrem, Endre Willassen, Elisabeth Stur. 2006-12-05. A comprehensive DNA sequence library is essential for identification with DNA barcodes.. https://doi.org/10.1016/j.ympev.2006.11.021

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