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PubMed · 17214051

Vitrification of mouse oocyte using open pulled straws compared with needles.

Surachai Pornwiroon·Somboon Kunathikom·Orawan Makemaharn·Rattanaphorn Huanaraj
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Abstract

OBJECTIVE: To compare the survival rate of mouse oocytes and fertilization rate between using open pulled straws (OPS) and needles for vitrification. MATERIAL AND METHOD: Meiosis II oocytes from female C57B/6J mice aged 7-8 weeks were collected and allocated to two groups for vitrification by using OPS or needles. Vitrified oocytes were thawed, morphological survival and fertilization rate were examined. RESULTS: There was no obvious difference between the morphological survival rates of vitrified mouse oocytes using OPS and needles (66.7% vs 64.8%). Proportions Difference 1.9% (95% CI -7.1, 10.7). The vitrified oocytes from the needle had significantly higher percentages of fertilization rate than OPS (76.8% vs 62.5%). Proportions Difference -14.3% (95% CI -24.5, -3.6). CONCLUSION: Vitrification method of mouse oocytes using needles when compared to OPS provides a similar morphological survival rate and higher fertilization rate.

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AnimalsCryopreservationFemaleMeiosisMiceMice, Inbred C57BLNeedlesOocytesComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Surachai Pornwiroon, Somboon Kunathikom, Orawan Makemaharn, Rattanaphorn Huanaraj. 2006. Vitrification of mouse oocyte using open pulled straws compared with needles.. https://pubmed.ncbi.nlm.nih.gov/17214051/

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