An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta.

John Wang; Stephanie Jemielity; Paolo Uva; Yannick Wurm; Johannes Gräff; Laurent Keller

doi:10.1186/gb-2007-8-1-r9

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An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta.

John Wang·Stephanie Jemielity·Paolo Uva·Yannick Wurm·Johannes Gräff·Laurent Keller

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Abstract

Ants display a range of fascinating behaviors, a remarkable level of intra-species phenotypic plasticity and many other interesting characteristics. Here we present a new tool to study the molecular mechanisms underlying these traits: a tentatively annotated expressed sequence tag (EST) resource for the fire ant Solenopsis invicta. From a normalized cDNA library we obtained 21,715 ESTs, which represent 11,864 putatively different transcripts with very diverse molecular functions. All ESTs were used to construct a cDNA microarray.

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Animals Ants Base Sequence Behavior, Animal Clone Cells Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Insect Longevity Molecular Sequence Data Oligonucleotide Array Sequence Analysis RNA, Viral Sequence Analysis, DNA Species Specificity Journal Article Research Support, Non-U.S. Gov't

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John Wang, Stephanie Jemielity, Paolo Uva, Yannick Wurm, Johannes Gräff, Laurent Keller. 2007. An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta.. https://doi.org/10.1186/gb-2007-8-1-r9

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