How does ppGpp affect translational accuracy in the stringent response?

A M Rojas; M Ehrenberg

doi:10.1016/0300-9084(91)90028-y

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How does ppGpp affect translational accuracy in the stringent response?

A M Rojas·M Ehrenberg

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Abstract

With an in vitro poly(Phe) synthesis system we have tested recent models concerning translational accuracy in the stringent response during aminoacid starvation. We have found that cognate, deacylated tRNA of very high concentrations is unable to block the A-site. No influence of EF-Tu.ppGpp on ribosomal proofreading has been found. Alternative mechanisms to keep translational errors low by the stringent response are discussed.

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Amino Acids Escherichia coli Guanosine Tetraphosphate Peptide Biosynthesis Peptide Elongation Factor Tu Peptides Protein Biosynthesis RNA, Bacterial RNA, Transfer, Phe Ribosomes Journal Article Research Support, Non-U.S. Gov't

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A M Rojas, M Ehrenberg. 1991. How does ppGpp affect translational accuracy in the stringent response?. https://doi.org/10.1016/0300-9084(91)90028-y

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