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Decrease in tetanic tension elicited by beta-adrenergic stimulation.

R Napolitano·L Vittone·C Mundiña·G Chiappe de Cingolani·A Mattiazzi
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Abstract

The effect of beta-adrenergic stimulation on tetanic tension (TT), maximal rate of rise of tension (+TT) and phospholamban (PHL) phosphorylation were studied in the perfused rat heart. 3 x 10(-8) M isoproterenol perfused at different [Ca2+]o 0.25, 1.35 and 3.85 mM, significantly decreased TT while increased +TT and PHL phosphorylation at the three [Ca2+]o studied. Regression lines of the relationship between +TT and TT from individual data obtained at each [Ca2+]o in the presence and in the absence of isoproterenol, show that for the same level of +TT, TT is lower in the presence of isoproterenol, i.e. at high levels of PHL phosphorylation. The slopes of the lines were 0.137 s and 0.427 s (P less than 0.05) in the presence and absence of isoproterenol respectively. The decrease in TT produced by the beta-agonist can be attributed to its relaxant action prevailing over its inotropic effect and may represent the mechanical expression of the enhanced phosphorylation of phospholamban.

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AnimalsCalciumCalcium-Binding ProteinsElectric StimulationIsoproterenolMyocardial ContractionPhosphorylationRatsRats, Inbred StrainsReceptors, Adrenergic, betaRyanodinePhospholambanJournal ArticleResearch Support, Non-U.S. Gov't

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R Napolitano, L Vittone, C Mundiña, G Chiappe de Cingolani, A Mattiazzi. 1991. Decrease in tetanic tension elicited by beta-adrenergic stimulation.. https://doi.org/10.3109/13813459109146941

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