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PubMed · 1723713

[The structure and function of botulinum type C neurotoxin].

K Kimura
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Abstract

The structure gene for botulinum type C neurotoxin was cloned from the toxigenic bacteriophage obtained from Clostridium botulinum type C, and the whole nucleotide sequence was determined. The nucleotide sequence contained a single open reading frame coding for 1,291 amino acids corresponding to a polypeptide with a molecular weight of 149,000. The signal peptide was not found after the first methionine residue. Upstream of the ATG codon, sequences predicted as a Shine-Dalgarno and a promoter were found. When the deduced amino acid sequence of type C toxin was compared with those of type A and D botulinum toxins and tetanus toxin, type C toxin shared about 52% identity with type D toxin, but shared only about 33% identity with type A and tetanus toxins. The structure and function of type C toxin were estimated from the results of epitope map with monoclonal antibodies and DNA thermal stability map.

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Amino Acid SequenceBase SequenceBotulinum ToxinsCodonDNA, BacterialEpitopesMolecular Sequence DataMolecular WeightEnglish AbstractJournal Article

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K Kimura. 1991. [The structure and function of botulinum type C neurotoxin].. https://pubmed.ncbi.nlm.nih.gov/1723713/

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